My spouse and i am producing in support of Janet Lerner’s app for an NSF Fellowship. Janet is usually genuinely a gifted college student with wonderful potential being a research science tecnistions. I inspire you to offer her the most consideration.
Janet has become conducting an independent honor’s study in my clinical for the past season. Without a doubt, Jesse is some of those rare individuals that comes along only one time every couple of years; she is remarkably intelligent (her transcript can be blemished by a single “B), inquisitive, enthusiastic, and creative.
Janet has just the right combination of assertiveness and respect to make her a joy to work with in the lab. She takes directions very well, but she is not timid about questioning experimental details or rationale. This is a student that I will enjoy watching develop into a highly productive research scientist.
For her honor’s thesis I have given Janet a demanding project that I would normally reserve for my graduate students.
Even before entering my lab, she had read a large number of articles that described recent advances with the virus that we study in the lab”JC virus (JCV). Expecting that I would need to explain many of the techniques used in these studies and to discuss the rationale behind the experiments, I was surprised to discover that she already had a firm grasp of these concepts and was ready to discuss the science on a much higher level. Together we decided that her project would entail a mutational analysis of a specific functional domain of the major regulatory protein of JCV, the multifunctional T protein.
To emphasize the scope and importance of Janet’s work, a summary of the relevant science follows. JCV is an important opportunistic pathogen; it has infected >70% with the world’s inhabitants and that remains valuable in the kidneys and minds of most people. In seriously immunocompromised people, JCV might cause the perilous demyelinating human brain disease modern multifocal leukoencephalopathy (PML); PML is now the cause of death in up to 7% of HELPS patients. A crucial question relevant to the pathogenesis of PML revolves around the mechanisms of latency and reactivation of JCV in the body. Manyof the earlier research have pointed to the To protein being a major person in these phenomena. We now think that this necessary protein contributes to the restricted web host specificity and inefficient GENETICS replicating activity of the computer virus.
Based upon assist the related monkey computer virus SV40, we now have lately begun to focus our attention on a zinc ring finger domain of the JCV Big t protein. This domain is usually thought to may play a role in forming a dual hexameric framework of Capital t that makes it possible for appropriate connection with virus-like DNA sequences at the duplication origin. We feel that variations in the consensus sequence in the JCV To protein zinc finger influences its capability to efficiently replicate the viral genome and to establish latency. Letter to Recommend Jesse Lerner “2
To investigate this kind of possibility, Jeremy is utilizing a modified Kunkel method of site-specific mutagenesis to convert the JCV T protein for an SV40-like healthy proteins within the finger region. Once constructed, these kinds of mutants will probably be tested in cell tradition for viability, promotion of DNA duplication, and oligomerization. Janet has turned excellent progress on her project. She has subcloned the appropriate explode of JCV DNA to a phagemid, validated its structure, and efficiently completed the mutagenesis reactions using control reagents.
Through the latter pair of experiments, many difficulties had been encountered. Jeremy demonstrated impressive resourcefulness in overcoming these kinds of problems. The lady did not just run to me for answers, but instead called a technical representative on the company that the reactants for the mutagenesis reactions were obtained. She also traveled to a graduate student inside the lab for more information. When she arrived at me to describe the problems, your woman was also ready to proffer solutions. Furthermore, Janet suggested additional settings to ensure that her ideas had been viable. Though I presented some alternative approaches, Jeremy was able to influence me that her strategy was better. She has the mutagenesis procedure functioning smoothly, and she has attemptedto produce her first mutants.
This strive yielded several potential mutants, but sadly sequence analysis (shegot the dideoxy solution to work wonderfully the initially time) suggested that they symbolized wild type clones. Undaunted, she has returned to the table, modified the annealing conditions for the mutagenic base and theme and is seeking again. Her perseverance and mature frame of mind in the face of a scientific set-back are uncommon for this kind of a young investigator. I have without a doubt that Janet will have a considerable influence upon our exploration program and this her efforts will result in the first authorship on an essential manuscript.
It must be apparent that I think extremely highly of Janet Lerner’s academic and research expertise. I are not alone in this evaluation. This wounderful woman has fit in very well with my personal research group (10 people including analysis assistant, postdoc, and graduate student and undergraduate students). The other undergraduates look up to her as a leader, and the graduate student students and postdoc interact with her while an equal. In conclusion, I may believe that you could find a more certified candidate intended for an NSF Fellowship than Janet Lerner. She has my strongest advice.